Phenoxyalkylcarboxylic acids

ABSTRACT

Arylcarbonylphenoxyalkylcarboxylic acids, and alkyl esters and salts thereof, are described. The compounds may be formulated into therapeutic compositions and administered to animals, including humans, for the treatment of hyperlipidaemia and related diseases.

This application is a continuation of Application Ser. No. 415,181 filed Sept. 7, 1982, which is a continuation of application Ser. No. 151,769, filed May 21, 1980, now abandoned, which is a divisional of application Ser. No. 042,156, filed May 24, 1979, now U.S. Pat. No. 4,238,492.

DESCRIPTION OF INVENTION

This invention relates to derivatives of phenoxylakylcarboxylic acids which can have useful therapeutic activity, for example as hypolipidaemic and hypocholesterolaemic agents.

The novel derivatives are particularly suitable for the treatment of cardiovascular ailments, especially atheromatic illnesses and illnesses related to a high level of lipids.

The compounds of this invention are of the formula ##STR1## wherein A is pyridyl, phenyl or phenyl substituted by one, 2 or 3 substituents independently selected from fluorine, chlorine, bromine, trifluoromethyl, nitro, hydroxy, C₁₋₄ alkyl and C₁₋₄ alkoxy;

R₁ and R₂ are the same or different and are each hydrogen or methyl;

R₃ is hydrogen or C₁₋₄ alkyl;

m is an integer of from one to 5;

Z₁ and Z₂ are the same or different and are each hydrogen, chlorine or methyl; and

the A--CO-- and --O--(CH₂)_(m) --CR₁ R₂ --COOR₃ groups are in the relative meta or para positions.

The present invention also provides the salts of the compounds of formula I, especially (i) the salts obtained by reaction with an organic or inorganic base when R₃ is hydrogen, and (ii) the acid addition salts obtained by reaction with an organic or inorganic acid (such as hydrochloric, oxalic, fumaric or maleic acid) when A is 2-pyridyl, 3-pyridyl or 4-pyridyl.

The preferred compounds of this invention are those wherein A is 3-pyridyl, phenyl, phenyl substituted by one or more Cl, Br or F atoms or CF₃, CH₃ or OCH₃ l groups (e.g. p-chlorophenyl), m is 3 or 4 and R₁ and R₂ are each methyl. It is often preferred that R₃,Z₁ and Z₂ are each hydrogen. A is most preferably 3-pyridyl. m is preferably 3 or 4.

A pharmaceutical composition according to the invention comprises a compound or non-toxic salt of the invention in association with a physiologically acceptable excipient. Such compositions can be used for the treatment of cardiovascular ailments.

The compounds of formula I may be prepared by known methods. The preferred method comprises reacting a hydroxyphenyl ketone of the formula ##STR2## with a halogenated ester of the formula

    Hal--(CH.sub.2).sub.m --CR.sub.1 R.sub.2 --COOR.sub.4

wherein A, Z₁, Z₂, m, R₁ and R₂ are as defined above, Hal is a halogen atom (preferably chlorine or bromine) and R₄ is C₁₋₄ alkyl (preferably methyl, ethyl, propyl, isopropyl, butyl, isobutyl or tert-butyl) in an anhydrous solvent (preferably dimethylformamide) in the presence of an alkali metal carbonate (preferably K₂ CO₃), to obtain a compound of formula I in which R₃ is C₁₋₄ alkyl. This ester may be hydrolysed, if desired, to give the corresponding acid where R₃ is hydrogen.

The following non-limitative Examples illustrate how compounds of formula I may be prepared. DMF means dimethylformamide.

EXAMPLE 1 Methyl 5-[4-(p-chlorobenzoyl)phenoxy]-2,2-dimethylpentanoate

116 g (0.5 mole) of 4'-chloro-4-hydroxybenzophenone, 84 g (0.6 mole) of potassium carbonate and 500 cm³ of DMF are placed in a 1 liter flask. The mixture is brought to reflux and 90 g (0.5 mole) of methyl 5-chloro-2,2-dimethylpentanoate in 200 cm³ of DMF are then added from a dropping funnel. Reflux is continued for 3 hours. The mixture is then cooled and poured into 1 liter of water, so that the desired product precipitates. The precipitate is extracted with diethyl ether, washed with aqueous soda, then with water, dried and decolourised, and the solvent is evaporated under vacuum. 160 g of a white solid are obtained, which are recrystallised from diisopropyl ether. 130 g of the desired product are obtained, m.pt. 64° C.

Analysis: Calcd C,67.29%; H,6.18% Found C,67.37%; H,6.29%

EXAMPLE 2 5-[4-(p-chlorobenzyl)phenoxy]-2,2-dimethylpentannoic acid

The ester of Example 1 is saponified with methanol containing soda to give the desired acid, m.pt. 134° C.

Analysis: Calcd C,66.57%; H,5.87% Found C,66.54%; H,5.81%

EXAMPLE 3 Methyl 5-[4-(p-chlorobenzoyl)-3,5-dimethylphenoxy]-2,2-dimethylpentanoate

Following the procedure of Example 1, but using 4'-chloro-3,5-dimethyl-4-hydroxybenzophenone as the starting ketone, the desired product is obtained as an oil in 71% yield.

EXAMPLE 4 5-[4-(p-chlorobenzoyl)-3,5-dimethylphenoxy]-2,2-dimethylpentanoic acid

The ester of Example 3 is saponified with methanol containing soda to give the desired acid, m.pt 135° C.

Analysis: Calcd C,67.94%; H,6.48% Found C,67.69%; H,6.40%

Table I records a number of products of formula I (in which R₁ and R₂ are each methyl) which have been prepared following the general procedure of the preceding Examples. The positions of the A--CO,Z₁ and Z₂ groups are given with respect to the --O--(CH₂)_(m) --CR₁ R₂ --COOR₃ group being arbitrarily defined as at the 1-position of the benzene ring. For instance, in Example 3, A is 4-(p-chlorophenyl), Z₁ is 3--CH₃ and Z₂ is 5--CH₃.

                  TABLE I                                                          ______________________________________                                         Ex-                                         m.pt.                              ample A             Z.sub.1 Z.sub.2                                                                              m   R.sub.3                                                                              (°C.)                       ______________________________________                                          5    4-(p-chlorophenyl)                                                                           2-CH.sub.3                                                                             5-CH.sub.3                                                                           3   CH.sub.3                                                                             (Oil)                               6    4-(p-chlorophenyl)                                                                           2-CH.sub.3                                                                             5-CH.sub.3                                                                           3   H      92                                 7    4-(p-chlorophenyl)                                                                           H       H     4   iso-  (Oil)                                                                    propyl                                    8    4-(p-chlorophenyl)                                                                           H       H     4   H     107                                 9    4-(3-pyridyl) H       H     3   CH.sub.3                                                                             --                                 10    4-(3-pyridyl) H       H     3   H     126                                11    4-phenyl      2-Cl    6-Cl  4   C.sub.2 H.sub.5                                                                      --                                 12    4-phenyl      2-Cl    6-Cl  4   H     --                                 13    4-(m-trifluoro-                                                                              H       H     3   CH.sub.3                                                                              70                                      methyl)phenyl                                                            14    4-(m-trifluoro-                                                                              H       H     3   H     110                                      methyl)phenyl                                                            15    4-(p-bromophenyl)                                                                            H       H     3   CH.sub.3                                                                              80                                16    4-(p-bromophenyl)                                                                            H       H     3   H     145                                17    4-(p-fluorophenyl)                                                                           H       H     4   iso-  (Oil)                                                                    propyl                                   18    4-(p-flourophenyl)                                                                           H       H     4   H     120                                19    3-phenyl      H       H     3   CH.sub.3                                                                             (Oil)                              20    3-phenyl      H       H     3   H      90                                21    3-(p-chlorophenyl)                                                                           H       H     3   CH.sub.3                                                                             (Oil)                              22    3-(p-chlorophenyl)                                                                           H       H     3   H      86                                23    3-(3',4'-dichloro-                                                                           H       H     4   CH.sub.3                                                                             --                                       phenyl)                                                                  24    3-(3',4'-dichloro-                                                                           2-CH.sub.3                                                                             5-CH.sub.3                                                                           4   CH.sub.3                                                                             --                                       phenyl)                                                                  25    3-(p-methoxyphenyl)                                                                          H       H     3   C.sub.4 H.sub.9                                                                      --                                 26    3-(m-tolyl)   H       H     3   C.sub.2 H.sub.5                                                                      --                                 27    3-(3-pyridyl) H       H     3   CH.sub.3                                                                             --                                 28    3-(3-pyridyl) H       H     3   H     --                                 ______________________________________                                    

The following is a summary of the pharmacological tests which have been carried out.

Male Wistar rats (250 to 300 g in weight) were divided into four groups of 20 animals each (2 experimental and 2 control groups). The rats were tested for 15 hours before the beginning of the experiment and throughout the tests. At the beginning of the experiment, the animals received, orally, 50 mg/kg of the tested compound in an aqueous gum suspension (gum arabic at a concentration of 30 g/l in water). The decreases in cholesterol level and of total lipids were measured after 24 and 48 hours from administration and the percentage decrease was calculated by comparison with the control rats (which did not receive the gum suspension). The results are shown in Table II.

                  TABLE II                                                         ______________________________________                                                                   Cholesterol                                          Example No.                                                                               Lipid Variation (%)                                                                           Variation (%)                                        of Compound                                                                               24 Hours 48 Hours  24 Hours                                                                              48 Hours                                  ______________________________________                                         10         -37      -53       -35    -31                                        8           0      -36       -18.8 -23.2                                      20           -24.8  -24       -18.8  -23.2                                     22         -35      -45       -30    -25                                       ______________________________________                                    

The compounds of the invention may be administered to man, orally or by injection.

In the form of capsules, the active substance is mixed with magnesium stearate, amigel and an excipient such as lactose. After filing, capsules are obtained which may, for example, each contain 200 mg of the active ingredient.

In the form of sweetened or unsweetened tablets, the active substance is mixed with lactose and icing sugar. The mixture is granulated in a fluidised bed and the granules which are obtained are tableted in the presence of magnesium stearate.

In injectable form, a soluble compound of formula I is used, for example a sodium salt, and the aqueous solution of this salt, for example in a concentration of 250 g/l is filtered and lyophilised under sterile conditions. The lyophilisate is introduced into a bottle in an amount of from 200 to 500 mg per bottle, ready for putting in extemporaneous solution, with sterile physiological serum, e.g. an aqueous solution of sodium chloride at a concentration of, say, 5 g/l.

Clinically, good results can be obtained with patients suffering from hyperlipidaemia by the administration of 200 to 500 mg daily of a compound of the invention over 20 hours. The most interesting products from a therapeutic view point are those of Table II and the most effective among these compounds are the products of Examples 8 and 22. 

I claim:
 1. A compound selected from the group consisting of those of formula I ##STR3## wherein A is selected from the group consisting of phenyl and phenyl substituted by 1, 2, or 3 substituents independently selected from the group consisting of fluorine, chlorine, bromine, trifluoromethyl, nitro, hydroxy, C₁₋₄ alkyl and C₁₋₄ alkoxy;R₁ and R₂ are each methyl; R₃ is selected from the group consisting of hydrogen and C₁₋₄ alkyl; m is 3 or 4; Z₁ and Z₂ are the same or different and are each selected from the group consisting of hydrogen, chlorine, and methyl; and the A--CO-- and --O--(CH₂)_(m) --CR₁ R₂ --COOR₃ groups are in the relative meta or para positions; and non-toxic salts thereof.
 2. A compound of claim 1 wherein R₁ and R₂ are each methyl.
 3. A compound of claim 1 wherein A is selected from the group consisting of phenyl and phenyl substituted by 1 or more Cl, Br, F, CF₃, CH₃ or OCH₃ groups; R₁ and R₂ are each methyl; and m is 3 or
 4. 4. A compound of claim 1 which is 6-[4-(p-chlorobenzoyl)phenoxy]-2,2-dimethylhexanoic acid.
 5. A compound of claim 1 which is 5-(3-benzoylphenoxy)-2,2-dimethylpentanoic acid.
 6. A compound of claim 1 which is 5-[3-(p-chlorobenzoyl)phenoxy]-2,2-dimethylpentanoic acid.
 7. A compound of claim 1 which is 5-[4-(p-chlorobenzoyl)phenoxy]-2,2-dimethylpentanoic acid.
 8. A compound of claim 1 which is 5-[4-(chlorobenzoyl)-3,5-dimethylphenoxy]-2,2-dimethylpentanoic acid.
 9. A compound of claim 1 which is 5-[4-(p-chlorobenzoyl)-2,5-dimethylphenoxy]-2,2-dimethylpentanoic acid.
 10. A compound according to claim 1 which is methyl 5[4-(p-chlorobenzoyl)phenoxy]-2,2-dimethylpentanoate.
 11. A compound according to claim 1, which is 5-[4-(m-trifluoromethylbenzoyl)phenoxyl]-2,2-dimethylpentanoic acid.
 12. A compound according to claim 1 which is 5-[4-(benzoyl)phenoxy]-2,2-dimethylpentanoic acid.
 13. A compound according to claim 1 which is 5-[4-(p-chlorobenzoyl)phenoxy]-2,2-dimethylpentanoic acid.
 14. A compound according to claim 1 which is 6-[4-(p-chlorobenzoyl)phenoxyl]-2,2-dimethylhexanoic acid.
 15. A compound according to claim 1, which is isopropyl 6-[4-(p-chlorobenzoyl)phenoxy]-2,2-dimethylhexanoate.
 16. A therapeutic composition useful in the treatment of hyperlipidaemia which comprises a therapeutically effective amount of a compound or salt of claim 1 and a physiologically acceptable excipient.
 17. A method for treating hyperlipidaemia which comprises administering to a patient suffering from pyperlipidaemia a therapeutically effective amount of a compound or salt of claim
 14. 